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Arsenite oxidation in batch reactors with alginate‐immobilized ULPAs1 strain
Author(s) -
Simeonova Diliana D.,
Micheva Kalina,
Muller Daniel A.E.,
Lagarde Florence,
Lett MarieClaire,
Groudeva Veneta I.,
Lièvremont Didier
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20530
Subject(s) - arsenite , oxidizing agent , arsenic , brine , chemistry , calcium alginate , environmental remediation , batch reactor , nuclear chemistry , environmental chemistry , contamination , calcium , organic chemistry , catalysis , biology , ecology
Arsenic is one of the major groundwater contaminants worldwide. It was previously demonstrated that the β‐proteobacterium Cenibacterium arsenoxidans has an efficient As[III] oxidation ability. The present study was conducted to evaluate the performance of alginate‐immobilized ULPAs1 in the oxidation of As[III] to As[V] in batch reactors. A two‐level full factorial experimental design was applied to investigate the influence of main parameters involved in the oxidation process, i.e., pH (7–8), temperature (4°C–25°C), kind of nutrient media (2%–20% sauerkraut brine), and arsenic concentration (10–100 mg/L). One hundred milligram per liter of As[III] was fully oxidized by calcium‐alginate immobilized cells in 1 h. It was found that the temperature as well as the kind of nutrient media used were significant parameters at a 95% confidence interval whereas only temperature was a significant parameter at a 99% confidence interval. The immobilization of the As[III] oxidizing strain in alginate beads offers a promising way to implement new treatment processes in the remediation of arsenic contaminated waters. © 2005 Wiley Periodicals, Inc.