Premium
Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension
Author(s) -
Lewis Miranda C.,
MacArthur Ben D.,
Malda Jos,
Pettet Graeme,
Please Colin P.
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20508
Subject(s) - oxygen tension , biological system , cell growth , oxygen , scaffold , diffusion , tension (geology) , oxygen transport , growth rate , limiting oxygen concentration , biochemical engineering , biophysics , work (physics) , mechanics , chemistry , computer science , materials science , biology , thermodynamics , mathematics , biochemistry , engineering , physics , geometry , composite material , organic chemistry , ultimate tensile strength , database
This article investigates heterogeneous proliferation within a seeded three‐dimensional scaffold structure with the purpose of improving protocols for engineered tissue growth. A simple mathematical model is developed to examine the very strong interaction between evolving oxygen profiles and cell distributions within cartilaginous constructs. A comparison between predictions based on the model and experimental evidence is given for both spatial and temporal evolution of the oxygen tension and cell number density, showing that behaviour for the first 14 days can be explained well by the mathematical model. The dependency of the cellular proliferation rate on the oxygen tension is examined and shown to be similar in size to previous work but linear in form. The results show that cell‐scaffold constructs that rely solely on diffusion for their supply of nutrients will inevitably produce proliferation‐dominated regions near the outer edge of the scaffold in situations when the cell number density and oxygen consumption rate exceed a critical level. Possible strategies for reducing such non‐uniform proliferation, including the conventional methods of enhancing oxygen transport, are outlined based on the model predictions. Copyright © 2005 Wiley Periodicals, Inc.