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Temperature‐triggered purification of antibodies
Author(s) -
Kim JaeYoung,
Mulchandani Ashok,
Chen Wilfred
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20451
Subject(s) - antibody , chemistry , precipitation , robustness (evolution) , selectivity , monoclonal antibody , combinatorial chemistry , biochemistry , biophysics , biology , immunology , catalysis , physics , meteorology , gene
In this article the unique capability of elastin‐like protein (ELP) to reversibly precipitate was combined with the high affinity and specificity of antibody‐binding domains such as Protein G, Protein L, or Protein LG as a general method for antibody purification that combines in a unique manner the simplicity and robustness of temperature‐triggered precipitation with the selectivity of affinity interactions. In a single precipitation step, antibodies derived from different sources (animal sera or hybridoma cell cultures) were selectively recovered by a simple temperature trigger. Due to the versatility of the binding ligands toward different classes of antibodies, we believe that this technology will be useful as an economical, highly efficient, and universal platform for the purification of antibodies. © 2005 Wiley Periodicals, Inc.