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High‐efficiency protein expression mediated by enterovirus 71 internal ribosome entry site
Author(s) -
Lee JinChing,
Wu TzongYuan,
Huang ChienFu,
Yang FengMine,
Shih ShinRu,
Hsu John T.A.
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20440
Subject(s) - internal ribosome entry site , ribosome , biology , messenger rna , translation (biology) , enterovirus 71 , virology , microbiology and biotechnology , virus , rna , protein biosynthesis , picornavirus , enterovirus , gene , genetics
An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23‐fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector. © 2005 Wiley Periodicals, Inc.