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Immobilization of single‐stranded DNA by self‐assembled polymer on gold substrate for a DNA chip
Author(s) -
Taira Shu,
Yokoyama Kenji
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20407
Subject(s) - dna , substrate (aquarium) , polymer , chemistry , nanotechnology , materials science , biochemistry , biology , organic chemistry , ecology
We developed a self‐assembling polymer based on polyallylamine (PAH) for use in DNA chips. Thioctic acid (TA) was covalently attached to PAH in sidechains to immobilize the polymer on a gold surface by self‐assembly. N ‐hydroxysuccinimide‐ester terminated probe single‐stranded (ss) DNA is easily covalently immobilized onto a TA‐PAH‐coated gold surface. Finally, the surface was covered with polyacrylic acid, which formed ion complexes with the TA‐PAH, to reduce the cationic charge. This ssDNA on a polymer‐coated surface recognized a fully matched DNA sequence and restrained nonspecific adsorption of target DNA. The selectivity and efficiency of hybridization was affected by adjusting the ionic strength of sodium chloride. © 2005 Wiley Periodicals, Inc.