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Biodiversity analysis of microbial community in the chem‐bioflocculation treatment process
Author(s) -
Xia Siqing,
Wang Feng,
Fu Yigang,
Yang Dianhai,
Ma Xingmao
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20339
Subject(s) - biodiversity , process (computing) , microbial population biology , biochemical engineering , ecology , chemistry , environmental science , biology , computer science , bacteria , engineering , genetics , operating system
Abstract Total DNA was directly extracted from environmental samples and amplified with polymerase chain reaction (PCR) technique. The PCR products were fingerprinted via denaturing gradient gel electrophoresis (DGGE). Significant differences were observed in the microbial community structures between traditional treatment process and chem‐bioflocculation process. The microbial community structure shift at different sampling locations in chem‐bioflocculation process and on two typical operational conditions was studied. 16S rDNA V3 regions of some dominant species were sequenced and the species were identified. The microbial communities were stable in both the chem‐bioflocculation process and the activated sludge process under various experimental conditions presented in this work. The attached growth treatment process was less stable when operational conditions changed. © 2005 Wiley Periodicals, Inc.