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Hydrolytic resolution of ( R,S )‐naproxen 2,2,2‐trifluoroethyl thioester by Carica papaya lipase in water‐saturated organic solvents
Author(s) -
Ng ISon,
Tsai ShauWei
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20314
Subject(s) - thioester , chemistry , lipase , naproxen , hydrolysis , kinetic resolution , substrate (aquarium) , organic chemistry , enantioselective synthesis , enantiomeric excess , stereochemistry , enzyme , medicine , oceanography , alternative medicine , pathology , geology , catalysis
For the first time, the Carica papaya lipase (CPL) stored in crude papain is explored as a potential enantioselective biocatalyst for obtaining chiral acids from their racemic thioesters. Hydrolytic resolution of ( R,S )‐naproxen 2,2,2‐trifluoroethyl thioester in water‐saturated organic solvents is employed as a model system for studying the effects of temperature and solvents on lipase activity and enantioselectivity. An optimal temperature of 60°C, based on the initial rate of ( S )‐thioester and a high enantiomeric ratio (i.e., E ‐value defined as the ratio of initial rates for both substrates) of >100 at 45°C in isooctane, is obtained. Kinetic analysis, considering product inhibition and enzyme deactivation, is also performed, showing agreement between the experimental and best‐fit conversions for ( S )‐thioester. A comparison of the kinetic and thermodynamic behaviors of CPL and Candida rugosa lipase (CRL) in isooctane and cyclohexane indicates that both lipases are very similar in terms of thermodynamic parameters ΔΔ H and ΔΔ S , initial rate of ( S )‐substrate, and E ‐value when ( R,S )‐naproxen 2,2,2‐trifluoroethyl thioester or ester is employed as substrate. © 2004 Wiley Periodicals, Inc.