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Activation of lignin peroxidase in organic media by reversed micelles
Author(s) -
Kimura Masayuki,
Michizoe Junji,
Oakazaki Shinya,
Furusaki Shintaro,
Goto Masahiro,
Tanaka Hiroo,
Wariishi Hiroyuki
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20277
Subject(s) - chemistry , micelle , lignin , aqueous solution , pulmonary surfactant , degradation (telecommunications) , lignin peroxidase , solvent , critical micelle concentration , peroxidase , nuclear chemistry , organic chemistry , enzyme , biochemistry , telecommunications , computer science
Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2‐ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined in this study; however, LIP was catalytically active by being entrapped in the AOT reversed micellar solution. LIP activity in the reversed micelle was enhanced by optimizing either the preparation or the operation conditions, such as water content and pH in water pools of the reversed micelle and the reaction temperature. Stable activity was obtained in isooctane because of the stability of the reversed micelle. The optimal pH was 5 in the reversed micellar system, which shifted from pH 3 in the aqueous solution. The degradation reaction of several environmental pollutants was attempted using LIP hosted in the AOT reversed micelle. Degradation achieved after a 1‐h reaction reached 81%, 50%, and 22% for p ‐nonylphenol, bisphenol A, and 2,4‐dichlorophenol, respectively. This is the first report on the utilization of LIP in organic media. © 2004 Wiley Periodicals, Inc.