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Detection of biomolecular interaction between biotin and streptavidin on a self‐assembled monolayer using magnetic nanoparticles
Author(s) -
Arakaki Atsushi,
Hideshima Sho,
Nakagawa Takahito,
Niwa Daisuke,
Tanaka Tsuyoshi,
Matsunaga Tadashi,
Osaka Tetsuya
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20262
Subject(s) - streptavidin , monolayer , magnetic nanoparticles , biomolecule , substrate (aquarium) , biotin , nanotechnology , nanoparticle , materials science , chemistry , biochemistry , oceanography , geology
For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self‐assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer‐modified substrate. The reaction of streptavidin‐modified magnetic particles on the biotin‐modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer‐modified substrate with biomolecule‐modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses. © 2004 Wiley Periodicals, Inc.