Premium
Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles
Author(s) -
Persson Patrik,
Baybak Oxana,
Plieva Fatima,
Galaev Igor Y.,
Mattiasson Bo,
Nilsson Bernt,
Axelsson Anders
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20236
Subject(s) - pressure drop , capillary action , mass transfer , adsorption , chromatography , matrix (chemical analysis) , mixing (physics) , volumetric flow rate , diffusion , materials science , convection , drop (telecommunication) , chemistry , analytical chemistry (journal) , porosity , chemical engineering , mechanics , thermodynamics , composite material , telecommunications , physics , organic chemistry , quantum mechanics , computer science , engineering
A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. © 2004 Wiley Periodicals, Inc.