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Model‐based optimization of a conductive matrix enzyme electrode
Author(s) -
Wu Xiaoxi,
Detzel Christopher J.,
Van Wie Bernard J.,
Haarsma Sarah J.,
Kidwell David A.
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20233
Subject(s) - electrode , amperometry , matrix (chemical analysis) , glucose oxidase , immobilized enzyme , mass transfer , range (aeronautics) , electrical conductor , biological system , representation (politics) , chemistry , materials science , computer science , enzyme , chromatography , composite material , electrochemistry , organic chemistry , politics , political science , law , biology
A mathematical model has been developed to describe the mechanism for internal mass transfer and enzyme reaction kinetics of an amperometric conductive matrix enzyme electrode. The model is simplified and solved analytically to arrive at a representation for the response slope in the linear range as well as for the response time. This is the first time that the response time of an enzyme electrode is described by a mathematical model. Simulations give information on how the design parameters influence the performance of the electrode for a glucose oxidase catalyzed sensing reaction process. Based on this information, several designs were constructed and tested showing suitable agreement with theoretical predictions. Finally, an optimized electrode was designed and validated. © 2004 Wiley Periodicals, Inc.