Premium
Development characterization and use of a high‐performance enzymatic time‐temperature integrator for the control of sterilization process' impacts
Author(s) -
Guiavarc'h Yann,
Van Loey Ann,
Zuber François,
Hendrickx Marc
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20183
Subject(s) - isothermal process , sterilization (economics) , materials science , chemistry , integrator , retort , chromatography , analytical chemistry (journal) , process engineering , thermodynamics , computer science , computer network , physics , organic chemistry , bandwidth (computing) , monetary economics , economics , foreign exchange market , foreign exchange , engineering
A small sized single‐component enzymatic time temperature integrator (TTI) was developed. It consisted of glass beads coated with Bacillus licheniformis α‐amylase (BLA) and stabilizing additives in a dehydrated form. Post heating residual enzymatic activity was used as a response property of the TTI. Under isothermal conditions, different batches of the system were characterized by z TTI ‐values around 13.5°C in the temperature range 100‐130°C as well as by their ability to provide a response within 5 min after thermal processing. When used under non‐isothermal conditions in a model food (silicone spheres), the system allowed to measure process‐values zTTI F 121.1°C up to 60 min with an average error of 10.9%. The capabilities of the system were validated in a real solid/liquid food matrix sterilized by retorting. The combination of F TTI ‐values with heat transfer simulations based on finite difference calculations allowed for the determination of process values, which evaluated actual process‐values 10°C F 121.1°C up to 90 min with an average error of 11.4%. The good performances of the system as well as its easiness of preparation and use, make the latter a valuable biological device for thermal process assessment. © 2004 Wiley Periodicals, Inc.