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Continuous glucose monitoring and control in a rotating wall perfused bioreactor
Author(s) -
Xu Yuanhang,
Sun Jiliang James,
Mathew Grace,
Jeevarajan Antony S.,
Anderson Melody M.
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20141
Subject(s) - bioreactor , hydrogen peroxide , chromatography , continuous glucose monitoring , perfusion , stock solution , chemistry , glucose oxidase , biosensor , standard solution , biomedical engineering , biochemistry , materials science , insulin , biology , microbiology and biotechnology , medicine , organic chemistry , glycemic
Abstract A glucose control system consisting of a single in‐line glucose sensor, concentrated glucose solution, and computer hardware and software were developed. The system was applied to continuously control glucose concentrations of a perfusion medium in a rotating wall perfused vessel (RWPV) bioreactor culturing BHK‐21 cells. The custom‐made glucose sensor was based on a hydrogen peroxide electrode. The sensor continuously and accurately measured the glucose concentration of GTSF‐2 medium in the RWPV bioreactor during cell culture. Three sets of two‐point calibrations were applied to the glucose sensor during the 55‐day cell culture. The system first controlled the glucose concentration in perfusing medium between 4.2 and 5.6 mM for 36 days and then at different glucose levels for 19 days. A stock solution with a high glucose concentration (266 mM) was used as the glucose injection solution. The standard error of prediction (SEP) for glucose measurement by the sensor, compared to measurement by the Beckman glucose analyzer, was ±0.4 mM for 55 days. © 2004 Wiley Periodicals, Inc.