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Combined effect of electrical stimulation and cisplatin in HeLa cell death
Author(s) -
Manabe Masafumi,
Mie Masayasu,
Yanagida Yasuko,
Aizawa Masuo,
Kobatake Eiry
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20110
Subject(s) - hela , cisplatin , stimulation , apoptosis , chemistry , cancer cell , cancer research , cell , viability assay , medicine , cancer , chemotherapy , biochemistry
The combined effect of electrical stimulation and cisplatin administration on HeLa cells was investigated. The combination of electric potentials (−0.5 V to 0.5 V) with 10‐Hz frequency and 1000 ng/mL cisplatin decreased cancer cell viability by 32% and was more effective than either treatment given alone. Combined treatment with cisplatin and electrical stimulation also increased the number of apoptotic cells. It is shown that the efficacy of cisplatin was enhanced in electrically stimulated HeLa cells, and the addition of electrical stimulation amplified the chemotherapeutic effect of cisplatin in cervical cancer cells. © 2004 Wiley Periodicals, Inc.