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Lipid‐gel and poly(dimethylsiloxane) film to mimic bioaccumulation in adipocytes
Author(s) -
Viravaidya Kwanchanok,
Jan Edward,
Shuler Michael L
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20109
Subject(s) - bioaccumulation , adipocyte , chemistry , gelatin , kinetics , chromatography , metabolite , lipid droplet , lipid bilayer , biophysics , biochemistry , adipose tissue , organic chemistry , biology , membrane , physics , quantum mechanics
Abstract Bioaccumulation is an increasingly important consideration in validation studies of the safety and efficacy of potential drugs. Although an “adipocyte” cell line model has been proven successful to mimic the accumulation of naphthalene in adipocytes, the prolonged incubation time limits its use in high‐throughput studies and reduces reproducibility. In this investigation, naphthalene and naphthol accumulation and uptake kinetics of thin poly(dimethylsiloxane) (PDMS) film and lipid nanospheres suspended in a crosslinked gelatin gel (lipid‐gel) were compared with those of adipocytes. Unlike the PDMS film, the lipid‐gel can mimic the kinetics and extent of naphthalene accumulation in the adipocytes reasonably well. However, the lipid‐gel accumulated about twice as much naphthol as the adipocytes, suggesting that hydrophobicity/hydrophilicity of the metabolite may be an important factor in the accuracy of accumulation studies with the lipid‐gel. Nonetheless, the lipid‐gel system shows promise as an inexpensive, convenient, and reproducible fat mimic for bioaccumulation studies. © 2004 Wiley Periodicals, Inc.