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Physiological significance of the cytometric distribution of fluorescent yeasts after viability staining
Author(s) -
Bouchez J.C.,
Cornu M.,
Danzart M.,
Leveau J.Y.,
Duchiron F.,
Bouix M.
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20054
Subject(s) - fermentation , yeast , saccharomyces cerevisiae , viability assay , staining , biology , ethanol fermentation , flow cytometry , fluorescent staining , fluorescence , biochemistry , computational biology , biological system , cell , microbiology and biotechnology , genetics , physics , quantum mechanics
This article describes a new method for the early detection of alcoholic fermentation arrest. This methodology is based on the flow cytometric assessment of Saccharomyces cerevisiae yeasts stained with a carboxyfluorescein diacetate fluorescent viability probe. Multicomponent analysis of viable cell distribution constitutes a promising new tool to describe physiological and dynamic changes to heterogeneous viable populations during alcoholic fermentation, through its ability to discriminate between successful processes and those ending prematurely. This framework, which is based on the comparison of cytometric histogram descriptors' combinations that can be related to simple physiological significance comparison, quickly and simply, allows testing yeasts for their fermentation ability and can be used to detect any kind of viability loss so that fermentation arrest can be avoided. © 2004 Wiley Periodicals, Inc.