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Isolation of a fluffy mutant of Aspergillus niger from chemostat culture and its potential use as a morphologically stable host for protein production
Author(s) -
van de Vondervoort Peter J. I.,
Poulsen Bjarne R.,
Ruijter George J. G.,
Schuleit Tina,
Visser Jaap,
Iversen Jens J. L.
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20046
Subject(s) - chemostat , mutant , biology , aspergillus niger , bioreactor , microbiology and biotechnology , transformation (genetics) , strain (injury) , heterokaryon , bacteria , biochemistry , genetics , gene , botany , anatomy
Chemostat cultivation of Aspergillus niger and other filamentous fungi is often hindered by the spontaneous appearance of morphologic mutants. Using the Variomixing bioreactor and applying different chemostat conditions we tried to optimize morphologic stability in both ammonium‐ and glucose‐limited cultures. In most cultivations mutants with fluffy (aconidial) morphology became dominant. From an ammonium‐limited culture, a fluffy mutant was isolated and genetically characterized using the parasexual cycle. The mutant contained a single morphological mutation, causing an increased colony radial growth rate. The fluffy mutant was subjected to transformation and finally conidiospores from a forced heterokaryon were shown to be a proper inoculum for fluffy strain cultivation. © 2004 Wiley Periodicals, Inc.