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Pediocin production by Pediococcus acidilactici in solid state culture on a waste medium: Process simulation and experimental results
Author(s) -
Vázquez J.A.,
González M.P.,
Murado M.A.
Publication year - 2004
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20018
Subject(s) - pediococcus acidilactici , piston (optics) , pulp and paper industry , chemistry , food science , productivity , solid state , process engineering , environmental science , chemical engineering , biological system , materials science , biology , bacteria , physics , lactic acid , engineering , genetics , macroeconomics , wavefront , optics , economics , lactobacillus plantarum
The production of pediocin by Pediococcus acidilactici was comparatively studied in submerged and solid‐state culture, using polyurethane foam particles soaked in commercial (MRS) and waste media with various supplements, where product concentrations were 15 times higher in MRS medium. For the solid state analysis, cultures were treated by successive compression and refilling of tubular minireactors equipped with a piston, without the need for reinoculation. This method was found to be simple, reproducible, and easily controllable, allowing culture productivity to be maintained for long periods of time without alterations in the basic properties of the system. In addition, yields were found to be superior compared to those from submerged culture. The system kinetics were modeled on the basis of widely accepted assumptions with a good fit to the experimental results and observed biomass fluctuations less evident than those predicted by the kinetic model. © 2004 Wiley Periodicals, Inc.