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In situ product removal of ketoses by immobilized 3‐amino phenyl boronic acid: Effect of immobilization method on pH profile
Author(s) -
Dukler Avinoam,
Freeman Amihay
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1160
Subject(s) - chemistry , phenylboronic acid , xylose , ketose , boronic acid , isomerization , organic chemistry , combinatorial chemistry , aldose , in situ , glyoxal , fructose , amino acid , catalysis , glycoside , biochemistry , fermentation
Abstract The use of polymeric derivatives of phenylboronic acid (PBA) as an effective means for specific in situ product removal of ketoses from aldose‐containing reaction mixtures, strongly depends on the retention of selective binding of ketoses exhibited by soluble PBA and 3‐amino PBA, by their polymeric, water insoluble analogs. In this communication we demonstrate that immobilization chemistry has a strong effect on ketose preferred binding by polymeric PBA derivatives. Our results indicate that for the preparation of an effective and more specific adsorbent, 3‐amino PBA should be coupled to the polymeric carrier via alkylamino chemistry and not via the commonly employed amido derivative. Immobilized alkylamino‐PBA exhibited selective fructose and xylulose binding throughout glucose and xylose isomerization processes at the pH range of 7.0–8.0. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 25–28, 2001.