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Stoichiometric growth model for riboflavin‐producing Bacillus subtilis
Author(s) -
Dauner Michael,
Sauer Uwe
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1153
Subject(s) - stoichiometry , bacillus subtilis , riboflavin , flux (metallurgy) , biological system , yield (engineering) , growth rate , thermodynamics , chemistry , composition (language) , metabolic flux analysis , biology , biochemistry , mathematics , physics , metabolism , organic chemistry , genetics , geometry , linguistics , philosophy , bacteria
Rate equations for measured extracellular rates and macromolecular composition data were combined with a stoichiometric model to describe riboflavin production with an industrial Bacillus subtilis strain using errors in variables regression analysis. On the basis of this combined stoichiometric growth model, we explored the topological features of the B. subtilis metabolic reaction network that was assembled from a large amount of literature. More specifically, we simulated maximum theoretical yields of biomass and riboflavin, including the associated flux regimes. Based on the developed model, the importance of experimental data on building block requirements for maximum yield and flux calculations were investigated. These analyses clearly show that verification of macromolecular composition data is important for optimum flux calculations. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 132–143, 2001.