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Biocatalysis of isomerization of 1,2‐benzisoxazole by bovine serum albumin in an organic solvent
Author(s) -
Liu XiaoChuan,
LemmaGray Patrizia
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1144
Subject(s) - chemistry , bovine serum albumin , isomerization , catalysis , solvent , biocatalysis , kinetics , serum albumin , aqueous solution , acetonitrile , organic chemistry , chromatography , reaction mechanism , biochemistry , physics , quantum mechanics
The catalytic properties of bovine serum albumin (BSA) have been studied rather extensively. The protein has shown catalytic activities toward a variety of reactions in aqueous media. However, there appears to be no reports of reactions catalyzed exclusively in organic media. In this study, we report the catalytic property of BSA on the isomerization of 1,2‐benzisoxazole in an organic solvent. Kinetics were performed using a continuous flow system in a spectrophotometer at 330 nm. The catalysis showed a Michaelis‐Menten behavior with an estimated catalytic efficiency ( k cat / K M ) of 8.77 M −1 S −1 . The influence of water content and inhibitors was also studied. The activity of BSA in acetonitrile is approximately 20% of that in water under similar conditions. Inhibition studies show that the active site may be the pyridoxal binding site involving Lys‐220. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 524–527, 2001.