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Bromoperoxidase activity in microplantlet suspension cultures of the macrophytic red alga Ochtodes secundiramea
Author(s) -
Rorrer Gregory L.,
Tucker Mary P.,
Cheney Donald P.,
Maliakal Sanjiv
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1129
Subject(s) - photobioreactor , photosynthesis , cell growth , chromatography , metabolism , botany , stationary phase , photoperiodism , bioreactor , chemistry , halogenation , light intensity , algae , biochemistry , biology , biophysics , ecology , organic chemistry , biofuel , physics , optics
Bromoperoxidase is an enzyme found in marine macroalgae that catalyzes the bromination of organic substrates. Photosynthetic microplantlet suspension cultures derived from the macrophytic red alga Ochtodes secundiramea were shown to possess bromoperoxidase. The optimum pH for O. secundiramea bromoperoxidase activity in cell‐free extracts was 6.0, and the half‐saturation constant for bromination of the exogeneous substrate monochlorodimedone (MCD) was 18 μ M . O. secundiramea microplantlets were cultivated in a bubble‐column photobioreactor at an incident light intensity of 38 μE m −2 s −1 (71% of light‐saturated photosynthesis, 10:14 light:dark photoperiod), and the kinetics of cell growth and bromoperoxidase production were followed. At these conditions, the specific growth rate was 0.052 day −1 . The lowest specific bromoperoxidase activity of 0.3 μmol MCD g −1 cell min −1 occurred during the midexponential phase of growth, and then increased steeply to 1.9 μmol MCD g −1 cell min −1 during the late stationary phase, suggesting that bromoperoxidase production was part of secondary metabolism. The estimated bromoperoxidase content in the cell mass at late stationary phase was 67 μg g −1 dry cell mass, demonstrating that bioreactor production of marine bromoperoxidase is feasible. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 389–395, 2001.