Premium
Immobilization of Candida rugosa lipase on colloidal gas aphrons (CGAs)
Author(s) -
O'Connell P. J.,
Varley J.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1116
Subject(s) - lipase , candida rugosa , chemistry , pulmonary surfactant , substrate (aquarium) , colloid , enzyme , chromatography , adsorption , biochemistry , biology , organic chemistry , ecology
A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10–100 μm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs. Leakage of protein from the CGAs and the activity of the adsorbed lipase decreased with increasing enzyme loading; this indicates that multilayers of lipase may be adsorbing onto the CGAs. The CGA‐immobilised lipase displayed normal Michaelis‐Menten dependence on substrate concentration and also exhibited greater activity than the free enzyme. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 264–269, 2001.