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Biosynthesis of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate‐ co ‐3‐hydroxyhexanoate) by metabolically engineered Escherichia coli strains
Author(s) -
Park Si Jae,
Ahn Woo Suk,
Green Phillip R.,
Lee Sang Yup
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1097
Subject(s) - escherichia coli , aeromonas hydrophila , biosynthesis , chemistry , monomer , aeromonas , polyhydroxyalkanoates , bacteria , recombinant dna , strain (injury) , biochemistry , gene , microbiology and biotechnology , food science , organic chemistry , biology , polymer , genetics , anatomy
The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3‐hydroxybutyrate (3HB), 3‐hydroxyvalerate (3HV), and 3‐hydroxyhexanoate (3HHx) [P(3HB‐ co ‐3HV‐ co ‐3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 81–86, 2001.