Biosynthesis of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate‐ co ‐3‐hydroxyhexanoate) by metabolically engineered  Escherichia coli  strains | Zendy

Park Si Jae | Zendy; Ahn Woo Suk | Zendy; Green Phillip R. | Zendy; Lee Sang Yup | Zendy

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Biosynthesis of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate‐ co ‐3‐hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Author(s) -

Park Si Jae,

Ahn Woo Suk,

Green Phillip R.,

Lee Sang Yup

Publication year - 2001

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.1097

Subject(s) - escherichia coli , aeromonas hydrophila , biosynthesis , chemistry , monomer , aeromonas , polyhydroxyalkanoates , bacteria , recombinant dna , strain (injury) , biochemistry , gene , microbiology and biotechnology , food science , organic chemistry , biology , polymer , genetics , anatomy

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3‐hydroxybutyrate (3HB), 3‐hydroxyvalerate (3HV), and 3‐hydroxyhexanoate (3HHx) [P(3HB‐ co ‐3HV‐ co ‐3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 81–86, 2001.

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