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Determination of 3‐deoxy‐D‐ arabino ‐heptulosonate 7‐phosphate productivity and yield from glucose in Escherichia coli devoid of the glucose phosphotransferase transport system
Author(s) -
Báez José Luis,
Bolívar Francisco,
Gosset Guillermo
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1088
Subject(s) - pep group translocation , yield (engineering) , escherichia coli , phosphotransferase , biochemistry , d glucose , biology , glucose transporter , chemistry , enzyme , microbiology and biotechnology , insulin , materials science , metallurgy , gene
The effect of inactivation of the glucose phosphotransferase transport system (PTS) on 3‐deoxy‐D‐ arabino ‐heptulosonate 7‐phosphate (DAHP) productivity and yield from glucose in Escherichia coli is reported. Strains used in this study were the PTS + PB103 and its PTS − glucose + derivative NF9. Their aroB − derivatives PB103B and NF9B were constructed to allow accurate measurement of total carbon flow into the aromatic pathway. The measured specific rates of DAHP synthesis were 0.55 and 0.94 mmol/g‐dcw · h and the DAHP molar yields from glucose were 0.43 and 0.71 mol/mol for the PTS + aroB − and the PTS − glucose + aroB − strains, respectively. For the latter strain, this value represents 83% of the maximum theoretical yield for DAHP synthesis from glucose. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 530–535, 2001.