Premium
A perspective of metabolic engineering strategies: moving up the systems hierarchy
Author(s) -
Bulter Thomas,
Bernstein Jeffrey R.,
Liao James C.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10845
Subject(s) - metabolic engineering , constructive , hierarchy , synthetic biology , biochemical engineering , systems biology , flux (metallurgy) , metabolic network , computer science , field (mathematics) , computational biology , chemistry , engineering , biology , biochemistry , mathematics , process (computing) , organic chemistry , economics , pure mathematics , market economy , enzyme , operating system
Metabolic engineering has been established as an important field in biotechnology. It involves the analysis, design, and alteration of the stoichiometric network using sophisticated mathematical and molecular biology techniques. It allows for improvement of pathway kinetics by removing flux bottlenecks, balancing precursors, and recycling cofactors used to increase product formation. The next step in the systems hierarchy is the constructive manipulation of regulatory networks. As our understanding of regulation continues to expand rapidly, engineering of intracellular regulation will become an integral aspect of metabolic engineering. © 2003 Wiley Periodicals, Inc.