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Construction of high‐density bacterial colony arrays and patterns by the ink‐jet method
Author(s) -
Xu Tao,
Petridou Sevastioni,
Lee Eric H.,
Roth Elizabeth A.,
Vyavahare Narendra R.,
Hickman James J.,
Boland Thomas
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10768
Subject(s) - bacteria , bacterial colony , escherichia coli , inkwell , agar plate , jet (fluid) , agar , biology , biological system , materials science , physics , composite material , biochemistry , genetics , gene , thermodynamics
We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm 2 were obtained by directly ejecting Escherichia coli ( E. coli ) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program. © 2003 Wiley Periodicals, Inc.