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A novel method of simulating oxygen mass transfer in two‐phase partitioning bioreactors
Author(s) -
Nielsen David R.,
Daugulis Andrew J.,
Mclellan P. James
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10721
Subject(s) - mass transfer coefficient , mass transfer , chemistry , oxygen , solubility , phase (matter) , volume fraction , organic compound , volume (thermodynamics) , dispersion (optics) , bioreactor , analytical chemistry (journal) , chromatography , thermodynamics , organic chemistry , physics , optics
An empirical correlation, based on conventional forms, has been developed to represent the oxygen mass transfer coefficient as a function of operating conditions and organic fraction in two‐phase, aqueous–organic dispersions. Such dispersions are characteristic of two‐phase partitioning bioreactors, which have found increasing application for the biodegradation of toxic substrates. In this work, a critical distinction is made between the oxygen mass transfer coefficient, k L a , and the oxygen mass transfer rate. With an increasing organic fraction, the mass transfer coefficient decreases, whereas the oxygen transfer rate is predicted to increase to an optimal value. Use of the correlation assumes that the two‐phase dispersion behaves as a single homogeneous phase with physical properties equivalent to the weighted volume‐averaged values of the phases. The addition of a second, immiscible liquid phase with a high solubility of oxygen to an aqueous medium increases the oxygen solubility of the system. It is the increase in oxygen solubility that provides the potential for oxygen mass transfer rate enhancement. For the case studied in which n ‐hexadecane is selected as the second liquid phase, additions of up to 33% organic volume lead to significant increases in oxygen mass transfer rate, with an optimal increase of 58.5% predicted using a 27% organic phase volume. For this system, the predicted oxygen mass transfer enhancements due to organic‐phase addition are found to be insensitive to the other operating variables, suggesting that organic‐phase addition is always a viable option for oxygen mass transfer rate enhancement. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 735–742, 2003.