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Lipase‐mediated acidolysis of butteroil with free conjugated linoleic acid in a packed bed reactor
Author(s) -
Sehanputri Prima S.,
Hill Charles G.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10720
Subject(s) - conjugated linoleic acid , packed bed , lipase , chemistry , conjugated system , chromatography , linoleic acid , organic chemistry , biochemistry , fatty acid , enzyme , polymer
Abstract Acidolysis of butteroil with free conjugated linoleic acid (CLA) was studied in a packed bed reactor containing an immobilized Candida antarctica (fraction B) lipase. Kinetic data were used to develop quantitative reversible rate expressions of the general Michaelis–Menten form that also incorporate a term for first‐order deactivation of the enzyme. The extent of incorporation of CLA in the triacylglycerols of butteroil was characterized for reactions carried out at several temperatures (namely 45°, 50°, and 55°C) with different weight ratios of butteroil to CLA (namely 10:1 and 2:1). At the optimum operating temperature of 50°C, similar levels of incorporation of CLA (60% to 85%) were achieved at low space times (<3 h) for both 10:1 and 2:1 (w/w) ratios of butteroil to CLA. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 608–617, 2003.