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Scale‐up of Artemisia annua L. hairy root cultures produces complex patterns of terpenoid gene expression
Author(s) -
Souret Frédéric F.,
Kim Yoojeong,
Wyslouzil Barbara E.,
Wobbe Kristin K.,
Weathers Pamela J.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10711
Subject(s) - artemisia annua , terpenoid , artemisinin , bioreactor , biology , botany , biochemistry , sesquiterpene , chemistry , plasmodium falciparum , malaria , immunology
Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale‐up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model for studying terpenoid production. These cultures were examined in shake flasks and compared with cultures grown in two types of bioreactors, a mist reactor and a bubble column reactor, which provide very different environments for the growing roots. Mist reactors have been shown previously to result in cultures that produce significantly more artemisinin per gram fresh weight of culture, while bubble column reactors have produced greater biomass. We have compared expression levels of four key terpenoid biosynthetic genes: 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase (HMGR), 1‐deoxy‐ D ‐xylulose‐5‐phosphate synthase (DXS), 1‐deoxy‐ D ‐xylulose‐5‐phosphate reductoisomerase (DXR), and farnesyl diphosphate synthase (FPS) in the three culture conditions. In shake flasks we found that although all four genes showed temporal regulation, only FPS expression correlated with artemisinin production. Light also affected the transcription of all four genes. Although expression in reactors was equivalent to or greater than that of roots grown in shake flasks, no correlation was found between expression level within six different zones of each reactor and their respective oxygen levels, light, and root‐packing density. Surprisingly, transcriptional regulation of HMGR, DXS, DXR, and FPS was greatly affected by the position of the roots in each reactor. Thus, relying on a single reactor sample to characterize the gene activity in a whole reactor can be misleading, especially if the goal is to examine the difference between reactor types or operating parameters, steps essential in scaling up cultures for production. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 653–667, 2003.