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Scalable inoculation strategies for microcarrier‐based animal cell bioprocesses
Author(s) -
Dürrschmid M.,
Landauer K.,
Simic G.,
Blüml G.,
DoblhoffDier O.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10700
Subject(s) - microcarrier , bioreactor , fluidized bed , bioprocess , chinese hamster ovary cell , fermentation , cell culture , biology , chemistry , food science , botany , paleontology , genetics , organic chemistry
Scalability is a major demand for high‐yield, stable bioprocess systems in animal cell culture–based biopharmaceutical production. Increased yields can be achieved through high‐density cell culture, such as in the combination of microcarrier and fluidized bed bioreactor technology. To minimize inocula volume in industrial applications of fluidized bed fermentation systems, it is crucial to increase the bed volume in the reactor during the fermentation process. We tested scale‐up strategy for the production of recombinant human arylsulfatase B (ASB) enzyme used in enzyme replacement therapy in patients afflicted with mucopolysaccharidosis type VI (MPS VI). This enzyme was derived from Chinese hamster ovary (CHO) cells cultivated as adherent cell culture on Cytoline macroporous microcarriers (Amersham Biosciences, Uppsala, Sweden) using a Cytopilot Mini fluidized bed bioreactor (FBR; Amersham Biosciences, Vogelbusch, Austria). Both 1:2 expansion (herein referred to as the addition of fresh, not‐yet‐colonized microcarriers) and 1:6 expansion of the carrier bed were performed successfully; the cells restarted to proliferate for colonizing these newly added carriers; and the stability of the culture was not negatively affected. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 681–686, 2003.