Premium
Growth dynamics of Artemisia annua hairy roots in three culture systems
Author(s) -
Kim Yoo Jeong,
Weathers Pamela J.,
Wyslouzil Barbara E.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10685
Subject(s) - artemisia annua , sugar , laboratory flask , biomass (ecology) , bubble , yield (engineering) , growth rate , botany , bioreactor , chemistry , respiration , horticulture , biology , food science , agronomy , mathematics , thermodynamics , mechanics , physics , plasmodium falciparum , geometry , artemisinin , malaria , immunology
Abstract The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O 2 and CO 2 . Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 428–443, 2003.