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Micro‐assembly of functionalized particulate monolayer on C 18 ‐derivatized SiO 2 surfaces
Author(s) -
Huang Tom T.,
Geng Tao,
Akin Demir,
Chang WooJin,
Sturgis Jennifer,
Bashir Rashid,
Bhunia Arun K.,
Robinson J. Paul,
Ladisch Michael R.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10680
Subject(s) - biotinylation , streptavidin , microbead (research) , monolayer , polystyrene , adsorption , bovine serum albumin , chemistry , surface modification , chromatography , colloid , listeria monocytogenes , chemical engineering , materials science , biotin , polymer , biochemistry , organic chemistry , bacteria , biology , engineering , genetics
This work describes a simple approach to immobilize functionalized colloidal microstructures onto a C 18 ‐coated SiO 2 substrate via specific or non‐specific bio‐mediated interactions. Biotinylated bovine serum albumin pre‐adsorbed onto a C 18 surface was used to mediate the surface assembly of streptavidin‐coated microbeads (2.8 μm), while a bare C 18 surface was used to immobilize anti‐ Listeria antibody‐coated microbeads (2.8 μm) through hydrophobic interactions. For a C 18 surface pre‐adsorbed with bovine serum albumin, hydrophobic polystyrene microbeads (0.8 μm) and positively charged dimethylamino microbeads (0.8 μm) were allowed to self‐assemble onto the surface. A monolayer with high surface coverage was observed for both polystyrene and dimethylamino microbeads. The adsorption characteristics of Escherichia coli and Listeria monocytogenes on these microbead‐based surfaces were studied using fluorescence microscopy. Both streptavidin microbeads pre‐adsorbed with biotinylated anti‐ Listeria antibody and anti‐ Listeria antibody‐coated microbeads showed specific capture of L. monocytogenes , while polystyrene and dimethylamino microbeads captured both E. coli and L. monocytogenes non‐specifically. The preparation of microbead‐based surfaces for the construction of microfluidic devices for separation, detection, or analysis of specific biological species is discussed. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 416–427, 2003.