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Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation
Author(s) -
Dai XiaoPing,
Majumdar Sudipto,
Luo Robert G.,
Sirkar Kamalesh K.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10653
Subject(s) - chromatography , elution , ultrafiltration (renal) , permeation , membrane , volumetric flow rate , adsorption , fiber , chemistry , membrane technology , materials science , bead , microfiltration , composite material , biochemistry , physics , organic chemistry , quantum mechanics
Hollow fiber membranes and chromatographic resin beads are commonly employed in a variety of bioseparation processes. A new class of integrated separation devices is being studied in which the shell side of a hollow fiber device is filled with adsorbents/chromatographic resin beads. Such devices and the corresponding separation methods integrate feed broth clarification by the microfiltration/ultrafiltration membrane with bioproduct purification by the shell‐side resin beads either as an adsorbent or as beads in elution chromatography. A mathematical model has been developed for the prediction of the chromatographic behavior of such an integrated device. Simulations have been done to study the effects of axial dispersion, feed flow rate, water permeation rate, fiber packing density, and void fraction. Numerical solutions were obtained by solving the governing equations. This model can reasonably describe the concentration profiles as well as the breakthrough and elution behaviors in the integrated device. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 125–139, 2003.