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High level of expression of recombinant human granulocyte‐macrophage colony stimulating factor in transgenic rice cell suspension culture
Author(s) -
Shin YunJi,
Hong ShinYoung,
Kwon TaeHo,
Jang YongSuk,
Yang MoonSik
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10635
Subject(s) - recombinant dna , granulocyte macrophage colony stimulating factor , cell culture , heterologous , genetically modified rice , biology , heterologous expression , transgene , gene expression , microbiology and biotechnology , genetically modified crops , gene , in vitro , biochemistry , genetics
Recombinant human granulocyte‐macrophage colony stimulating factor (hGM‐CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM‐CSF accumulated in the culture medium dropped quickly from its maximum of 150 μg/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM‐CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 778–783, 2003.