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Potential of cell retention techniques for large‐scale high‐density perfusion culture of suspended mammalian cells
Author(s) -
Voisard D.,
Meuwly F.,
Ruffieux P.A.,
Baer G.,
Kadouri A.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10629
Subject(s) - context (archaeology) , perfusion , scale (ratio) , process engineering , centrifuge , microfiltration , materials science , biomedical engineering , biochemical engineering , chemistry , membrane , biology , engineering , physics , medicine , paleontology , biochemistry , quantum mechanics , nuclear physics , cardiology
Abstract This review focuses on cultivation of mammalian cells in a suspended perfusion mode. The major technological limitation in the scaling‐up of these systems is the need for robust retention devices to enable perfusion of medium as needed. For this, cell retention techniques available to date are presented, namely, cross‐flow filters, hollow fibers, controlled‐shear filters, vortex‐flow filters, spin‐filters, gravity settlers, centrifuges, acoustic settlers, and hydrocyclones. These retention techniques are compared and evaluated for their respective advantages and potential for large‐scale utilization in the context of industrial manufacturing processes. This analysis shows certain techniques have a limited range of perfusion rate where they can be implemented (most microfiltration techniques). On the other hand, techniques were identified that have shown high perfusion capacity (centrifuges and spin‐filters), or have a good potential for scale‐up (acoustic settlers and inclined settlers). The literature clearly shows that reasonable solutions exist to develop large‐scale perfusion processes. © 2003 Wiley Periodicals, Inc. Biotechol Bioeng 82: 751–765, 2003.