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RGD‐grafted poly‐l‐lysine‐ graft ‐(polyethylene glycol) copolymers block non‐specific protein adsorption while promoting cell adhesion
Author(s) -
VandeVondele Stephanie,
Vörös Janos,
Hubbell Jeffrey A.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10625
Subject(s) - protein adsorption , copolymer , polymer chemistry , peg ratio , adsorption , ethylene glycol , surface modification , chemistry , cell adhesion , polyethylene glycol , covalent bond , adhesion , biocompatibility , side chain , polystyrene , combinatorial chemistry , polymer , biochemistry , organic chemistry , finance , economics
A novel class of surface‐active copolymers is described, designed to protect surfaces from nonspecific protein adsorption while still inducing specific cell attachment and spreading. A graft copolymer was synthesized, containing poly‐(L‐lysine) (PLL) as the backbone and substrate binding and poly(ethylene glycol) (PEG) as protein adsorption‐resistant pendant side chains. A fraction of the grafted PEG was pendantly functionalized by covalent conjugation to the peptide motif RGD to induce cell binding. The graft copolymer spontaneously adsorbs from dilute aqueous solution onto negatively charged surfaces. The performance of RGD‐modified PLL‐ g ‐PEG copolymers was analyzed in protein adsorption and cell culture assays. These coatings efficiently blocked the adsorption of serum proteins to Nb 2 O 5 and tissue culture polystyrene while specifically supporting attachment and spreading of human dermal fibroblasts. This surface functionalization technology is expected to be valuable in both the biomaterial and biosensor fields, because different signals can easily be combined, and sterilization and application are straightforward and cost‐effective. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 784–790, 2003.