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Cyclic fed‐batch culture for production of human serum albumin in Pichia pastoris
Author(s) -
Bushell M. E.,
Rowe M.,
AvigRossa C. A.,
Wardell J. N.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10616
Subject(s) - chemostat , fed batch culture , glycerol , yield (engineering) , pichia pastoris , biomass (ecology) , chemistry , food science , albumin , biochemistry , fermentation , chromatography , biology , bacteria , recombinant dna , agronomy , genetics , materials science , gene , metallurgy
Simple cyclic fed‐batch culture (cfbc), consisting of a constant medium feed with periodic withdrawals of culture, resulted in a product yield (13.4 mg protein per gram biomass) similar to that obtained using the complex multiphase industrial production strategy (13.7 mg protein per gram biomass). In cfbc, productivity was ultimately limited by the rate at which the cells could assimilate methanol. Glycerol was inhibitory to growth at high concentrations. However, product yield continued to increase as the glycerol concentration was increased. In chemostat culture, dissolved oxygen concentration influenced product yield independently of any detectable influence on cell growth. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 678–683, 2003.