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Controling the rate of protein release from polyelectrolyte complexes
Author(s) -
Kamiya Noriho,
Klibanov Alexander M.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10606
Subject(s) - lysozyme , polyelectrolyte , chemistry , particle size , chemical engineering , particle (ecology) , biophysics , charge density , chromatography , polymer chemistry , crystallography , chemical physics , polymer , organic chemistry , biochemistry , biology , ecology , engineering , physics , quantum mechanics
Extended protein release from readily prepared, water‐insoluble complexes with oppositely charged polyions is explored. Using hen egg‐white lysozyme as a model, its sustained release from such complexes with a number of polyanions under physiological conditions has been demonstrated and rationalized. The rate of release varies orders of magnitude and is controlled by the nature of the polyanion (decreasing upon increase in its linear charge density, length, and hydrophobicity) and the complex particle size (the larger the particles, the slower the release). © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 590–594, 2003.