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Expression and secretion of the heterodimeric protein interleukin‐12 in plant cell suspension culture
Author(s) -
Kwon T. H.,
Seo J. E.,
Kim J.,
Lee J. H.,
Jang Y. S.,
Yang M. S.
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10528
Subject(s) - secretory protein , secretion , callus , cell culture , signal peptide , biology , microbiology and biotechnology , transgene , plant cell , protein subunit , biochemistry , peptide sequence , gene , botany , genetics
It has been suggested that plant cell culture is the most suitable system for producing small‐to‐medium quantities of specialized, expensive, and high‐purity proteins. Here, we report that a heterodimeric protein, human interleukin‐12 (hIL‐12), was expressed and secreted into culture medium in a biologically active form. A transgenic plant expressing hIL‐12 was constructed by sexual crossing of plants that expressed each subunit of the protein. From a piece of transgenic plant, callus was induced and cell suspension culture was established. The biological activity and amount of hIL‐12 secreted into culture medium were analyzed using bioassays and ELISA. Analysis of cellular localization demonstrated that the protein was secreted into the culture medium together with its intrinsic signal peptide. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 870–875, 2003.