Premium
Parameter scanning ultrafiltration: Rapid optimisation of protein separation
Author(s) -
Ghosh Raja,
Wan Yinhua,
Cui Zhanfeng,
Hale Geoff
Publication year - 2003
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10510
Subject(s) - ultrafiltration (renal) , fractionation , chromatography , chemistry , membrane , separation process , salt (chemistry) , bovine serum albumin , biochemistry
Abstract High‐resolution fractionation of proteins using ultrafiltration is feasible only at highly optimised conditions. Conventional process optimisation methodology demands both time and material. Pulsed sample injection ultrafiltration has been suggested as a rapid process optimisation technique. In the present work the scope of this technique is further extended by “parameter scanning ultrafiltration,” which involves continuous change of a process parameter (e.g., pH, salt concentration). The time and material consumption are thus further reduced. The technique was validated using different proteins and membranes. Sieving coefficients at different pH and salt concentration were compared to those obtained in fixed parameter ultrafiltration experiments. As fractionation case studies the separation of monoclonal antibody from bovine serum albumin and separation of human IgG from human serum albumin were examined. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 673–682, 2003.