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Cell‐Type‐Specific adhesion onto polymer surfaces from mixed cell populations
Author(s) -
Quirk Robin A.,
Kellam Barrie,
Bhandari Re.,
Davies Martyn C.,
Tendler Saul J. B.,
Shakesheff Kevin M.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10502
Subject(s) - asialoglycoprotein receptor , adhesion , cell adhesion , polylysine , biophysics , chemistry , cell , hepatocyte , hepatic stellate cell , biochemistry , biology , in vitro , organic chemistry , endocrinology
The targeted adhesion of a specific cell type from a mixed cell suspension via the surface presentation of a cell‐specific ligand is demonstrated. This generic strategy is illustrated by the covalent attachment of a galactose derivative to a polylysine backbone via the amine functionality. Following adsorption of the resultant material to a polymer surface, hepatocyte adhesion is increased via the interaction between galactose and asialoglycoprotein receptors in a concentration‐dependent manner. The selective nature of the material is demonstrated by the approximate doubling in the adhesion of hepatocytes relative to a nontargeted cell type (hepatic stellate cells), and an inability of the modified polymer surface to attract additional numbers of the nontargeted cells. This strategy provides a mechanism for controlling the ratios of cell types adhering to scaffold supports, thus enabling the rapid creation of defined coculture systems from heterogeneous cell suspensions. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 625–628, 2003.