Premium
Water‐in‐oil macroemulsions sustain long‐term viability of microbial cells in organic solvents
Author(s) -
Stefan Alessandra,
Palazzo Gerardo,
Ceglie Andrea,
Panzavolta Eleonora,
Hochkoeppler Alejandro
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10476
Subject(s) - escherichia coli , aqueous solution , chemistry , ternary operation , solvent , saccharomyces cerevisiae , homogeneous , mixing (physics) , food science , microorganism , lecithin , chromatography , microbiology and biotechnology , biology , bacteria , yeast , biochemistry , organic chemistry , physics , genetics , quantum mechanics , computer science , gene , programming language , thermodynamics
Abstract Extremely stable water‐in‐oil macroemulsions have been obtained by dispersing water in isooctane in the presence of lecithin. Either prokaryotic ( Escherichia coli ) and eukaryotic ( Saccharomyces cerevisiae and Rhodotorula minuta ) cells hosted in these water‐in‐oil macroemulsions are viable for weeks despite the consistent excess of organic solvent (ranging from 70 to 84%, v/v) in these ternary systems. Conjugation occurs upon mixing macroemulsions containing F + or F − Escherichia coli strains, indicating consistent mass transfer between the water droplets. Populations of yeasts hosted in water‐in‐oil macroemulsion feature a higher frequency of cells aggregation when compared with the corresponding populations suspended in homogeneous aqueous media. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 323–328, 2003.