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Biooxidation of n ‐hexanol by alcohol oxidase and catalase in biphasic and micellar systems without solvent
Author(s) -
KarraChaabouni Maha,
Pulvin Sylviane,
Meziani Abdelghani,
Thomas Daniel,
Touraud Didier,
Kunz Werner
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10452
Subject(s) - hexanol , chemistry , alcohol , chromatography , pulmonary surfactant , solvent , catalase , glycerol , aqueous solution , hexanal , yield (engineering) , bioconversion , organic chemistry , enzyme , biochemistry , materials science , fermentation , metallurgy
Alcohol oxidase from Pichia pastoris together with catalase from bovine liver was used to oxidize n ‐hexanol to hexanal. For this purpose, an aqueous buffer solution was mixed with large amounts of hexanol by simple agitation, yielding a biphasic system, or by adding the nonionic surfactant Brij 35. Initial velocities and reaction yields after 24 h were measured as a function of various parameters such as the amounts of enzymes, hexanol, or surfactant. High enzymatic activity was determined for hexanol concentrations of between 20 mass% and 80 mass% without using any additional organic solvent. The homogenization of the biphasic systems with the help of Brij 35 did not yield a significant improvement of the bioconversion, which would justify the use of surfactants. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 27–32, 2003.