Premium
Cell separation mediated by differential rolling adhesion
Author(s) -
Greenberg Adam W.,
Hammer Daniel A.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.1043
Subject(s) - cd34 , haematopoiesis , progenitor cell , bone marrow , adhesion , cell adhesion , cell , selectin , biology , stem cell , chemistry , microbiology and biotechnology , cell adhesion molecule , immunology , biochemistry , organic chemistry
Recently, we showed a correlation between the maturity of hematopoietic stem and progenitor cells during development and rolling efficiency on selectins. These findings motivated us to explore a novel separation that exploits differences in selectin‐mediated rolling adhesion between populations of cells. We extend the use of a previously developed cell‐free system to study the separation of populations of sialyl Lewis x (sLe x )‐coated microspheres designed to roll with different average velocities on L‐selectin chimeric substrates under well‐defined flow. Results show that a separation that exploits differences in average rolling velocities between cell or microsphere populations is attainable. Excellent recovery and purity values for the slower rolling, or more desirable, populations are obtained and can be estimated from rolling velocity measurements. We also assess the feasibility of a selectin‐mediated separation of adult bone marrow cell populations using previously obtained rolling velocity and rolling flux data for CD34 + and CD34 − adult bone marrow cells on L‐selectin substrates. We believe that a cell separation mediated by differential rolling adhesion can be used to enrich populations of hematopoietic stem and progenitor cells from an adult bone marrow cell preparation and that this method possesses several major advantages over existing antibody‐mediated cell‐affinity chromatography technologies. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 111–124, 2001.