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Inhibition of bacterial attachment by pulsed Nd:YAG laser irradiations: An in vitro study using marine biofilm‐forming bacterium Pseudoalteromonas carrageenovora
Author(s) -
Nandakumar Kanavillil,
Obika Hideki,
Shinozaki Tatsuya,
Ooie Toshihiko,
Utsumi Akihiro,
Yano Tetsuo
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10416
Subject(s) - biofilm , pseudoalteromonas , bacteria , microbiology and biotechnology , in vitro , marine bacteriophage , chemistry , biology , biochemistry , 16s ribosomal rna , genetics
The effect of low mean power laser irradiations with short pulse duration from an Nd:YAG (neodymium‐doped yttrium aluminium garnet) laser on a marine biofilm‐forming bacterium, Pseudoalteromonas carrageenovora , was investigated in the laboratory. Laser‐irradiated bacteria were tested for their ability to attach on nontoxic titanium nitride (TiN) coupons with nonirradiated bacteria as the reference. Two durations of irradiation were tested, 10 and 15 min. Bacterial attachment was monitored after 20 min, 40 min, and 1 h of irradiation. The average laser fluence used for this study was 0.1 J/cm 2 . The area of attachment of the irradiated bacteria was significantly less than the reference for both durations of irradiation. The growth of irradiated bacteria showed a longer lag phase than the nonirradiated sample, mainly due to mortality in the former. The bacterial mortality observed was 23.4 ± 0.71 and 48.6 ± 6.5% for 10‐ and 15‐min irradiations, respectively. Thus, the results show that low‐power pulsed laser irradiations resulted in a significant bacterial mortality and a reduced bacterial attachment on nontoxic hard surfaces. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 552–558, 2002.