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Correcting mass isotopomer distributions for naturally occurring isotopes
Author(s) -
Van Winden Wouter A.,
Wittmann Christoph,
Heinzle Elmar,
Heijnen Joseph J.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10393
Subject(s) - isotopomers , isotope , mass spectrometry , stable isotope ratio , chemistry , flux (metallurgy) , metabolic flux analysis , radiochemistry , analytical chemistry (journal) , chromatography , physics , nuclear physics , molecule , metabolism , biochemistry , organic chemistry
In one method of metabolic flux analysis, simulated mass spectrometry data is fitted to measured mass distributions of metabolites that are isolated from cultures with defined feeding of 13 C‐labeled substrates. Doing so, simulated mass distributions must be corrected for the presence of naturally occurring isotopes. A method that was recently introduced for this purpose consists of consecutive correction steps for each isotope of each element in the considered compound. Here we show that all isotopes of each individual element must, however, be corrected in one single step. Furthermore, it is shown that the source of information with respect to isotopic compositions of the elements needs to be chosen with care. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 477–479, 2002.