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Fabrication and use of a transient contractional flow device to quantify the sensitivity of mammalian and insect cells to hydrodynamic forces
Author(s) -
Ma Ningning,
Koelling Kurt W.,
Chalmers Jeffrey J.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10387
Subject(s) - laminar flow , mechanics , dissipation , microfluidics , transient (computer programming) , computational fluid dynamics , materials science , volumetric flow rate , flow (mathematics) , impeller , nanotechnology , physics , thermodynamics , computer science , operating system
A microfluidic device was fabricated via photolithographic techniques which can create transient elongational and shear forces ranging over three orders of magnitude while still maintaining laminar flow conditions. The contractional fluid flow inside the microfluidic device was simulated with FLUENT (a computational fluid dynamics computer program) and the local deformation forces were characterized with the scalar quantity, local energy dissipation rate. The sensitivities of four cell lines (CHO, HB‐24, Sf‐9, and MCF7) were tested in the device. The results indicate that all four cell lines are able to withstand relatively intense energy dissipation rates (up to 10 4 –10 5 kW/m 3 ), which is orders of magnitude higher than the maximum local energy dissipation rates generated by impellers in bioreactors, but comparable to that associated with small bursting bubbles. While the concept that suspended animal cells are relatively robust with respect to purely hydrodynamic forces in bioprocess equipment is well known, these results quantitatively demonstrate these observations. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 428–437, 2002.