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Separating lysozyme from bacteriophage P22 in two‐phase aqueous micellar systems
Author(s) -
Kamei Daniel T.,
King Jonathan A.,
Wang Daniel I. C.,
Blankschtein Daniel
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10377
Subject(s) - micelle , aqueous solution , sodium dodecyl sulfate , chemistry , lysozyme , phase (matter) , pulmonary surfactant , ethylene oxide , yield (engineering) , aqueous two phase system , bacteriophage , micellar solutions , chromatography , chemical engineering , organic chemistry , biochemistry , materials science , escherichia coli , copolymer , metallurgy , polymer , gene , engineering
This communication demonstrates that two‐phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two‐phase aqueous nonionic n ‐decyl tetra(ethylene oxide) (C 10 E 4 ) micellar system increases the yield of a model net positively charged protein, lysozyme, in the micelle‐rich phase from 75 to 95%, while still maintaining approximately the same yield of a model net negatively charged virus, bacteriophage P22, in the micelle‐poor phase (97% vs. 98%). © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 233–236, 2002.