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Advantages of continuous over batch reactors for the kinetic analysis of enzymes inhibited by an unknown substrate impurity
Author(s) -
Gallifuoco Alberto,
Alfani Francesco,
Cantarella Maria
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10326
Subject(s) - substrate (aquarium) , uncompetitive inhibitor , enzyme , kinetics , non competitive inhibition , chemistry , impurity , kinetic energy , enzyme kinetics , chromatography , stereochemistry , thermodynamics , biochemistry , active site , physics , organic chemistry , biology , ecology , quantum mechanics
A new experimental technique, employing a continuous stirred‐tank reactor, for studying enzyme kinetics in the presence of inhibitor‐contaminated substrate is described. The proposed method is simulated mathematically for competitive, uncompetitive, and mixed‐type noncompetitive inhibition. The step‐by‐step experimental procedure is described, as is the necessary data analysis for determining the kinetic parameters. Differences in system response for enzyme inhibition by excess substrate and by an impurity are illustrated, and a stability analysis of the system is performed. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 641–646, 2002.